Protran Blotting Membranes
Standard membranes for protein and nucleic acid blotting
- Standard membranes for protein and nucleic acid blotting
- Excellent sensitivity, resolution and low background
- Superior mechanical strength
- Compatible with a variety of assays and detection methods
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Protran® nitrocellulose membranes are the most frequently specified transfer media in the world for Western, Southern and Northern blotting. Additionally, the membrane is excellent for carbohydrate studies (Glycomics).
Protran membranes are manufactured using 100 % pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as “nitrocellulose“ may actually contain large amounts of cellulose acetate which will lower the protein binding capacity.
Protran® membranes have the best handling strength of all pure nitrocellulose membranes. They are compatible with a variety of detection methods, including isotopic, chemiluminescent (peroxidase/ luminol-based), colorimetric and fluorescent. Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wet in water, and then in the transfer buffer. No other pre-treatment steps are necessary.
The Protran supported membrane consists of pure 100 % nitrocellulose cast onto both sides of an inert polyester support material. The support in no way affects transfer conditions or results and gives the membrane exceptional handling characteristics, allowing it to be reprobed repeatedly.
Nitrocellulose, 100 % pure
80 – 90 μg/cm2
Approx. 60 – 80 μg/cm2
DNA and RNA
DNA and RNA
Backen (80 °C)
DNA und RNA
DNA und RNA
++ = recommended
+ = limited use
– = not recommended
* Use of horseradish peroxidase based systems is recommended.
|Order number||Item||Pore size||Dimension||Quantity/ Pack|
|846-B10401396*||Protran NC 0.2||0.20 µm||30 cm x 4 m||1 roll|
|846-B10401196*||Protran NC 0.45||0.45 µm||30 cm x 4 m||1 roll|
|846-B10401180*||Protran NC 0.45||0.45 µm||30 cm x 60 cm||5 sheets/pack|
|Protran NC 0.45||0.45 µm||33 cm x 56 cm||5 sheets/pack|
|846-B10439196*||Protran supported NC 0.45||0.45 µm||30 cm x 4 m||1 roll|
* Please note that the product is discontinued. A sale is only possible while stocks last.