innuPREP Proteinase K

innuPREP Proteinase K

Highly active recombinant protease from Pichia pastoris with endo- and exoproteolytic activity

Product Details

  • Contains no RNases or DNases, and virtually no DNA
  • Consistent quality and performance
  • Robust enzyme: stable over a broad pH range
  • Ideal for a diverse array of applications, such as preparing cell lysates for subsequent nucleic acid isolation

Proteinase K is one of the most active endopeptidases known. The enzyme is extraordinarily effective against native proteins and can be used for quickly inactivating endogenous RNases and DNases. Proteinase K is particularly suitable for isolating nucleic acids for use in amplification reactions, for isolating native RNA and DNA from tissues and cell lines, for promoting cell lysis by activating a bacterial autolysis factor, and for modifying proteins and/or glycoproteins on cell surfaces (for membrane structure analyses).

Inhibitors: None of the following inactivate the enzyme: metal ions, chelating agents (such as EDTA), sulfhydryl reagents, or trypsin and chymotrypsin inhibitors.

Activators: Proteinase K activity is stimulated by the presence of denaturing agents (SDS and urea).

Note: SDS can produce a seven-fold increase in Proteinase K activity.

Optimum pH: Proteinase K is stable over a broad pH range (4 to 12.5), and retains its full activity for several hours if incubated at a pH between 6.5 and 9.5.

The enzyme can reduce proteins to free amino acids if a large excess of protein is present and if incubated for long periods of time.

Specifications

Concentration

20 mg/mL at an activity of 20 U/mg

Quality Control

Proteinase K is lyophilized and purified via chromatography, after which it is tested to ensure that no RNases, DNases and exonucleases are present. These should not be detectable.

Order Information

Order numerQuantity
845-CH-00100066.0 mg (add 0.3 mL ddH2O)
845-CH-001003030.0 mg (add 1.5 mL ddH2O)

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MSDS innuPREP Proteinase K

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Manual innuPREP Proteinase K

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