innuPREP Gel Extraction Kit

innuPREP DOUBLEpure Kit

Combination kit for fast extraction of DNA fragments from agarose gels or amplification products from PCR reaction mixtures as well as sequencing reactions

Product Details

  • Flexible elution volumes between 30 and 50 µl (standard protocol) and 10 to 20 µl (»Mini-Elute« protocol)
  • High recovery rates of up to 95 %
  • Capable of processing fragment lengths of up to 30 kb

The innuPREP DOUBLEpure Kit allows efficiently extracting of DNA fragments from TAE or TBE agarose gels and utilizes a novel 2-step technology for purifying amplification products from PCR reaction mixtures and sequencing reaction mixtures. The Spin Filter column has a binding capacity of over 20 µg, making it possible to achieve high yields and excellent quality when isolating DNA fragments. In addition, the kit also produces recovery rates of up to 95 % depending on the fragment size. Other outstanding features of the innuPREP DOUBLEpure Kit include easy handling, flexible settings for elution volumes and fast purification protocols.

Specifications

Starting material

  • PCR reaction mixes (up to 50 µl)
  • TAE agarose gels (up to 300 mg)
  • TBE agarose gels (up to 300 mg)
  • Sequencing reaction mixture (up to 50 µl)

Average rate of return

  • PCR-purification: ca. 60 % – 95 %
  • Gel extraction: ca. 60 % – 90 %
  • Sequencing reaction purification: > 75 %
  • depending on fragment length

Extraction time

  • PCR purification: approx. 3 minutes (2-step process)
  • Gel extraction: approx. 20 minutes
  • Sequencing reaction purification: approx. 2 minutes (2-step process)

Fragment lengths

  • PCR-purification: > 60 bp – 30 kb
  • Gel extraction: 100 bp – 30 kb

Binding capacity

Column binding capacity: > 20 µg DNA

What Our Customers Says

"We ensure a maximum yield and handling is easier and more comfortable"

"With your kit we ensure a maximum yield of 95-100 %. Additionally the handling is earier and more comfortable – and easier to convey to our biology students during their internships."

Dr. Eva Steinmetz, PostDoc and staff member of the Institute for Developmental Biology at the Saarland University Hospital, Germany

Order Information

Order numberQuantity
845-KS-505001010 reactions
845-KS-505005050 reactions
845-KS-5050250250 reactions

Downloads

Manual innuPREP DOUBLEpure Kit

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SDS innuPREP DOUBLEpure Kit

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Short Manual innuPREP DOUBLEpure Kit - Protocol 1

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Short Manual innuPREP DOUBLEpure Kit - Protocol 2

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Short Manual innuPREP DOUBLEpure Kit - Protocol 3

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