Configuration examples for MALDI-TOF-MS sample preparation
Standard MALDI sample preparation
Capacity
- 1x MALDI plate
- 1-4x sample plate(s)
Throughput
- < 10 min to prepare a MALDI target plate
Start situation
- 1x Matrix reservoir
- 1x Tip wash station wash buffer
- 1x MALDI plate
- 1-4x source plate(s)
Extended standard MALDI sample preparation
Including plate storage
Capacity
- CyBio QuadStack M: 90 MALDI plates or 30 source plates (1536) and 30 MALDI plates or 40 source plates (384) and 10 MALDI plates
Throughput
- < 10 min to prepare a MALDI target plate
Start situation
- 1x Matrix reservoir
- 1x TWS wash buffer
- 1-30x MALDI plates
- 1-40x source plate(s)
Fully automated MALDI-TOF sample preparation
Including storage for source and MALDI plates
Capacity
- Plate storage - Hotel: 10 x 21 = 210 sample plates
- CyBio QuadStack M: 90 MALDI plates
Throughput
- < 10 min to prepare a MALDI target plate
Start situation
- 1x Matrix reservoir
- 1x TWS wash buffer
- 1-90 x MALDI plates
1-210 x source plate(s)
CB-Vario deck layout
- MALDI plate
- Source plate
- Matrix reservoir
- De-ionisation
- Tip touch station
- Tip wash
Extended fully automated MALDI-TOF sample preparation
Including plate store, sealer, peeler, centrifuge
Capacity per batch
- 90 MALDI target plates (CyBio QuadStack (M) 3 racks) and 180 (CyBio QuadStack (L) 3 racks) source plates
Throughput
- < 10 min to prepare a MALDI target plate
Start situation
- 1x Matrix reservoir
- 1x TWS wash buffer
- 1-90 x MALDI plates
- 1-180 x source plate(s)
MALDI-TOF reader plate delivery set up
Including CyBio QuadStack M
Capacity
- 1 - 90 plates
Throughput
- 10 s for plate handling
Start situation
- MALDI plates sorted in the CyBio QuadStack M
Including random accesses plate storage
Capacity
- 1 - 110 plates
Throughput
- 10 s for plate handling
Start situation
MALDI plates sorted in the shelf
Components
| Analytik Jena Devices | |
|---|---|
| Component | Description |
| CyBio® Well vario Pipettor | Base station without pipetting head; combine with a circular transport system and a pipetting head to form a functional system |
| 10 position circular transport system | Circular transport system with base frame and rotary table with 10 positions |
| CyBio® Well vario Pipetting head with 1536/8 μl or 384/25 μl Tip Magazine | CyBio® Well vario Pipetting Head for 384- or 1536-well simultaneous liquid handling and Fixed Tip Magazine 1536/1 µl or 384/25 µl Ceramic with individually changeable non-disposable ceramic tips |
| CyBio® Well vario Barcode reader NLV 3101 | For reading barcode labels on the short side of microplates on linear transport system |
| Pump module | Pump module with 2 pumps and power supply |
| Tip wash station 1536 | Complete tip wash station 1536 set |
| MALDI sample adapter 1536 | For optimal dry transfer of small volumes. Optimized for Bruker MALDI sample plate/MALDI microplates with 127.7 mm x 85.4 mm footprint |
| Microplate adapter 1536 | For use with 1536-well microplates with CyBio® Well vario |
| Reservoir 1536 | Optimized for applications with CyBio® Well vario Pipetting Head, 1536 with refillable option on request |
| Active dryer | Park deck and vacuum chamber to reduce the drying time for MALDI sample plate |
| CyBio® QuadStack M | Medium sized stacker with capacity for 30 SW plates per stack, MALDI sample plate storage |
| Third Party example devices | |
|---|---|
| Component | Description |
| MS reader | High speed mass spectrometer |
| Robotic arm | 4 Axis Scara robotic arm with 750 mm vertical stroke, servo gripper |
| Incubator | Storage of assay plates (optional with lid) with a capacity for 210 shallow well plates |
| Peeler | Fully automated seal removal in wide variety of environments |
| Sealer | Thermal microplate sealer, accommodates microplates as tall as 63.5 mm, sealing temperature 30 to 200°C, cycle time ~ 8 seconds per plate |
| Centrifuge | Micro plate centrifuge for up to 5000 x g, imbalance tolerant: up to 100 g weight difference between buckets, 2-bucket rotor, maximum plate height: 61 mm |
| Hotel | Carousel hotel package, 10 place, 220 capacity, package with 10 extended-height random access hotels |